Journal: bioRxiv

Article Title: Conditional Dystrophin ablation causes profound effects on muscle development, neurobehavior, and extracellular matrix pathways

doi: 10.1101/2025.01.30.635777

Figure Lengend Snippet: A. Schematic outlining the generation of myofiber-specific Dystrophin conditional knockout mice ( Dmd :HSA KO) by breeding our Dmd flox52 /Y mice to human skeletal actin ( HSA )-Cre transgenic mice. Cre-mediated excision of Dmd exon 52 results in an out-of-frame disruption and instability of the Dp427 RNA transcript. B. Western immunoblotting for the large Dp427m protein in the TA and soleus muscles of WT (open), Dmd flox52 /Y (blue), mdx 5cv (red), and Dmd :HSA KO (green) adult (6-month-old) male mice. C. Densitometry graphs WT, Dmd flox52 /Y , mdx 5cv , and Dmd :HSA KO immunoblots taken from TA and soleus muscle lysates and normalized to Vinculin protein. N = 5 separate mice per cohort. D. Histochemical staining of TA, soleus, and diaphragm muscles isolated from the WT, Dmd flox52 /Y , mdx 5cv , and Dmd :HSA KO male mice. Hematoxylin and eosin (H&E), picrosirius red, and Masson’s trichrome histochemical staining performed. Scale bar = 100 µm. E. Percent fibrosis (%) in the TA muscles of the four mouse cohorts analyzed. F. Percentage (%) centralized myonuclei for the approximately 600 myofibers of the TA muscles of the four mouse cohorts analyzed. G. Cross-sectional area (CSA) displaying myofiber sizes (µm 2 ) shown for each of the four cohorts. Two-way ANOVA with Bonferroni correction performed. P-values of significance are shown: * p < 0.05, ** p < 0.01, *** p < 0.005, and **** p < 0.001.

Article Snippet: Masson’s trichrome staining was performed using a Masson’s Trichrome Stain Kit (Polysciences, Inc; Warrington, PA; Cat# 25088-1) and picrosirius red staining was performed using a Picrosirius Red Stain Kit (Polysciences, Inc; Cat# 24901-500).

Techniques: Knock-Out, Transgenic Assay, Disruption, Western Blot, Muscles, Staining, Isolation